![]() ![]() The first group consists of assay of fibrinolytic activity with natural proteins as substrates, e.g., fibrin plate methods. Existing assay methods can be divided into three major groups. There are several assay methods for these enzymes this may due to the insolubility of substrate, fibrin. Microbial fibrinolytic enzymes, especially those from food-grade microorganisms, have the potential to be developed as functional food additives and drugs to prevent or cure thrombosis and other related diseases. The fibrinolytic enzymes were successively discovered from different microorganisms, the most important among which is the genus Bacillus. Microbial fibrinolytic enzymes have attracted much more attention than typical thrombolytic agents because of the expensive prices and the side effects of the latter. In general, there are four therapy options: surgical operation, intake of antiplatelets, anticoagulants, or fibrinolytic enzymes. All rights reserved.Īctivity assessment of microbial fibrinolytic enzymes.Ĭonversion of fibrinogen to fibrin inside blood vessels results in thrombosis, leading to myocardial infarction and other cardiovascular diseases. These results suggest that SOT presents important possibilities for application in the therapy of thrombosis. Results show that SOT and nattokinase have very different fibrinolytic and fibrinogenolytic modes, engendering significant synergetic effects of SOT and nattokinase on fibrinolysis. Furthermore, SOT had some plasminogen activator-like activity. ![]() The fibrinolytic activity of SOT is about 18-fold higher than that of plasmin, and is comparable to that of t-PA by fibrin plate assays. Compared to other enzymes, SOT showed remarkably higher hydrolytic activity toward mimic peptides of fibrin and plasminogen. The fibrinolytic activity of SOT was examined using in vitro assays and was compared with those of known fibrinolytic enzymes such as plasmin, tissue-type plasminogen activator (t-PA), urokinase, and nattokinase. We introduce a highly potent fibrinolytic serine protease from Streptomyces omiyaensis (SOT), which belongs to the trypsin family. Uesugi, Yoshiko Usuki, Hirokazu Iwabuchi, Masaki Hatanaka, Tadashi Highly potent fibrinolytic serine protease from Streptomyces. ![]()
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